Light-emitting crystals of aptamer-hybrid organic semiconductor signaling on human cells expressing EpCAM
- 주제(키워드) OLED-aptamer hybrid crystals , Specific binding aptamer , Cell recognition , Turn-on sensing
- 발행기관 한국공업화학회
- 발행년도 2022
- 총서유형 Journal
- DOI http://dx.doi.org/10.1016/j.jiec.2022.09.017
- KCI ID ART002907139
- 본문언어 영어
초록/요약
In this study, we successfully performed protein-cell recognition by combining an organic light-emittingdiode (OLED) material and an aptamer with a three-dimensional DNA structure. Tris (8-hydroxyquinoline) aluminum (Alq3), one of the most widely used green OLED components used in displays,was chosen as an emitter. Epithelial cell adhesion molecule (EpCAM) was selected as a targetbecause it is well-established in cancer studies. The generated micrometer-sized particles exhibitedincreased luminous intensity based on the specific recognition to proteins and cells on hybrid interfaces,proving that the aptamer maintained its three-dimensional structure while binding to its target. Interestingly, only human cells expressing EpCAMs were distinguishable, whereas mouse carcinoma cellswere not. Furthermore, use of graphene oxide as a quencher made these aptamer-Alq3 microrods 2-foldmore effective at enhancing the fluorescence signal upon interaction with human oral squamous carcinomacells. This new approach, which imparts biometric functionality to organic semiconductor materials,will provide a new platform for the evolution of OLED hybrid materials.
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