Study on the effects of the neuronal differentiation and anti-inflammation through ectopic expression of TIMP2 and/or Angiogenin genes
TIMP2 또는 Angiogenin 유전자의 과발현을 통한 신경 분화능과 항염증 효능에 관한 연구
- 주제(키워드) Stem cell Therapy , Neuronal Differentiation , Anti – inflammation , gene overexpression , Angiogenin , TIMP2
- 발행기관 고려대학교 대학원
- 지도교수 홍성회
- 발행년도 2025
- 학위수여년월 2025. 2
- 학위명 석사
- 학과 및 전공 대학원 의생명융합과학과
- 원문페이지 57 p
- 실제URI http://www.dcollection.net/handler/korea/000000292170
- UCI I804:11009-000000292170
- DOI 10.23186/korea.000000292170.11009.0001893
- 본문언어 영어
목차
ABSTRACT i
국문 초록 iii
TABLE OF CONTENTS v
LIST OF TABLES vi
LIST OF FIGURES vii
NOMENCLATURE viii
CHAPTER 1. INTRODUCTION 1
CHAPTER 2.MATHERIALS AND METHODS 4
CHAPTER 3.RESULTS 9
3.1 Identification and selection of TIMP2 and ANG as key factors secreted by neural stem cells. 9
3.2 Induced neural stem cells exhibit the characteristics of neural stem cells. 12
3.3 Overexpression of TIMP2 and ANG in iNSCs via lentiviral transduction. 14
3.4 Effect of TIMP2 and/or ANG overexpression on cell apoptosis and proliferation in iNSCs. 16
3.5 TIMP2 promotes neuronal differentiation and maturation of iNSCs. 18
3.6 Establishing an optimal inflammatory model to evaluate the anti-Inflammatory effects of TIMP2 and ANG overexpression. 31
3.7 Pro-inflammatory cytokine mRNA expression levels are reduced by iNSC conditioned media overexpressing TIMP2 and/or ANG. 34
3.8 Enhanced mRNA expression levels of anti-inflammatory cytokines by TIMP2 and/or ANG overexpressing conditioned media. 38
CHAPTER 4.DISCUSSION 40
REFERENCES 43
