Synthesis of sub-50 nm bio-inspired silica particles using a C-terminalmodified ferritin template with a silica-forming peptide
- 주제(키워드) Silica-forming peptide , Ferritin , Silicification , Biosilica nanoparticles , Drug delivery
- 발행기관 한국공업화학회
- 발행년도 2021
- 총서유형 Journal
- KCI ID ART002808902
- 본문언어 영어
초록/요약
Silica-forming peptides (SFPs) and the SFP-modified structural proteins can be used as templates for thesynthesis of biosilica nanoparticles (NPs). However, such biomolecule-mediated synthesis showed limitationsfor the generation of NPs with sizes sub-50 nm. In this study, the SFP sequences(KPSHHHHHTGAN and KPTHHHHHHDG for Kps and Kpt, respectively) were fused to the C-terminus ofthe human ferritin heavy chain (Fn), resulting in the SFP moieties in the inner space of Fn (termed FncKpsand Fn-cKpt). Using Fn-cSFP templates for silicification in two-phase system, the Fn-cKps@SiO2and Fn-cKpt@SiO2 NPs were generated in mean diameters of 26 and 28 nm, respectively. Also, weemployed biosilica NPs sub-50 nm for a doxorubicin (Dox) delivery system (application model). FncKpt@SiO2 NPs exhibited a high loading efficiency compared to Fn-cKpt only (1.7-fold) and prolongedrelease patterns with Dox. Most importantly, the uptake of Fn-cKpt@SiO2 into cancer cells was increasedso that the efficient delivery of Dox to the cell inside was observed. The uniform generation of biosilicaNPs sub-50 nm obtained here is a new achievement. Together with the ideal pH-dependent drug release,the size-controlled design of biosilica will be a useful strategy for efficient delivery of chemical drugs totarget cells.
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