Effects of Cellular Senescence on the Motile Patterns of Normal Cells
- 주제(키워드) Biophysics
- 발행기관 고려대학교 대학원
- 지도교수 이경진
- 발행년도 2022
- 학위수여년월 2022. 2
- 학위구분 석사
- 학과 대학원 물리학과
- 세부전공 비선형동력학 및 생물물리학전공
- 원문페이지 41 p
- UCI I804:11009-000000257302
- DOI 10.23186/korea.000000257302.11009.0001371
- 본문언어 영어
초록/요약
Collective cell migration is a main characteristic of many physiological phenomena, being of importance to a range of biological processes such as embryonic development, wound healing, immune action, and tumor metastasis. In those cases, cell-cell interactions might affect the motion of single cells, interfering with the collective dynamics. While aspects of the dynamics of single-cell organisms and homogenous populations of diverse cell types are largely studied, here we estimate the adhesion energy between normal (non-senescent) and senescent MDA-MB-231 cells. To address that, the results of numerical simulations of a model and that of experiments were combined, with special attention to the boundary interactions between inhomogeneities (senescent cells) and the normal cell population. Data was acquired through in vitro time-lapse imaging, in which the cells were kept under constant temperature (36 °C), and the images were consistently acquired with a time interval of one minute between frames. Given the unique morphology of senescent cells, we theorized that differences in interactions between normal cell-cell and senescent-normal-cell arrangements are expected. Several characteristics inherent to the later system were observed during our experiments and corresponding simulations. For instance, experimental results indicate that the angular velocity of normal tumor cells around senescent tumor cells and the senescent cell radii are inversely correlated. Additionally, for an estimated average value of angular velocity and radius, the simulations suggest that the adhesion energy is weaker between senescent-normal cells when compared to normal-normal cell doublets. Finally, the residence length showed to be a few times longer than the one of the normal cells in confluency, suggesting that the senescent cells would behave as a physical barrier in a confluent population.
more목차
1. Introduction 1
1.1 The Role of Cell Motility and Tissue Dynamics in Biological Phenomena 1
1.2 Cell Adhesion Energy 2
1.3 Cell Adhesion Dependent Population Dynamics 3
1.4 Cellular Senescence 4
1.5 MDA-MB-231 – A Highly Invasive Breast Cancer Cell Line 6
2. Methods 8
2.1 Cell Culture 8
2.2 Live-cell Imaging 9
2.3 The Cellular Potts Model Simulation 10
3. Experimental Results and Discussion 13
3.1 Motility of Contacting Cell-Pairs 16
3.2 Simulated Cell-Pair Interactions 19
3.3 Simulated Interactions in Confluent Population 25
4. Conclusion 28
5. References 31
