Caloric Restriction-Induced Extension of Chronological Lifespan Requires Intact Respiration in Budding Yeast
Caloric Restriction-Induced Extension of Chronological Lifespan Requires Intact Respiration in Budding Yeast
- 주제(키워드) caloric restriction , chronological lifespan , electron transport chain , mitochondria , respiration
- 발행기관 한국분자세포생물학회
- 발행년도 2017
- 총서유형 Journal
- KCI ID ART002224264
- 본문언어 영어
초록/요약
Caloric restriction (CR) has been shown to extend lifespan and prevent cellular senescence in various species ranging from yeast to humans. Many effects of CR may contribute to extend lifespan. Specifically, CR prevents oxidative damage from reactive oxygen species (ROS) by enhancing mitochondrial function. In this study, we characterized 33 single electron transport chain (ETC) gene-deletion strains to identify CR-induced chronological lifespan (CLS) extension mechanisms. Interestingly, defects in 17 of these 33 ETC gene-deleted strains showed loss of both respiratory function and CR-induced CLS extension. On the contrary, the other 16 respiration-capable mutants showed increased CLS upon CR along with increased mitochondrial membrane potential (MMP) and intracellular adenosine triphosphate (ATP) levels, with decreased mitochondrial superoxide generation. We measured the same parameters in the 17 non-respiratory mutants upon CR. CR sim-ultaneously increased MMP and mitochondrial superoxide generation without altering intra-cellular ATP levels. In conclusion, respiration is essential for CLS extension by CR and is important for balancing MMP, ROS, and ATP levels.
more