Direct observation of the formation of DNA triplexes by single-molecule FRET measurements
Direct observation of the formation of DNA triplexes by single-molecule FRET measurements
- 주제(키워드) DNA triplex , H-DNA , Single-molecule FRET , pH , Strand alignment
- 발행기관 한국물리학회
- 발행년도 2012
- 총서유형 Journal
- UCI G704-001115.2012.12.4.008
- KCI ID ART001682590
- 본문언어 영어
초록/요약
In this report we investigated the effects of various biological and chemical factors (DNA sequence, pH,ions, and molecularity) on the formation of DNA triplexes through single-molecule FRET technique. Using this method, we determined how the third strand bound to a DNA duplex and how stable the triplex structure was under various conditions. From this study, we not only verified a variety of well-known features of DNA triplex but also discovered or experimentally supported several interesting behaviors:at neutral pH, a pyrimidine-motif triplex can be formed; the parallel arrangement was not only possible but also dominant over the antiparallel arrangement for a purine-motif triplex. We demonstrated that our method is a versatile analytical tool in studying structural aspects of nucleic acids, particularly nonclassical DNA structures, and provides insights into physical mechanism of such structures.
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