배아줄기세포에 의한 영양세포의 지방 분화 : Adipogenic Differentiation of Feeder Cells by Human Embryonic Stem cells
Adipogenic Differentiation of Feeder Cells by Human Embryonic Stem cells
- 주제(키워드) human embryonic stem cells , adipogenesis , feeders , mouse embryonic fibrobalsts , human skin fibroblasts , human msenchymal stem cells
- 발행기관 한국조직공학과 재생의학회
- 발행년도 2010
- 총서유형 Journal
- UCI G704-SER000000617.2010.7.1.002
- KCI ID ART001424646
- 본문언어 한국어
초록/요약
Human embryonic stem(hES) cells have generally been cultured on mouse embryonic fibroblast feeder cells. Paracrine factors from feeder cells have drawn much attention in their relation to the maintenance of selfrenewal and pluripotency of hES cells. There has however been no attention on factors from hES cells and their effects on feeder cells. In the present study, we examined the effect of hES cells on feeder cells, mouse embryonic fibroblasts, human mesenchymal stem cells and human skin fibroblasts, in indirect co-culture system using porous membrane. We observed lipid droplet formation in feeder cells as early as 2 days after co-culture and lipid vacuole matured with time in the influence of hESCs. Only with hES-conditioned medium adipogeneisis of feeder cells occurred but co-culture with hES cells dramatically increased adipogenesis of feeder cells. This implied adipogenesis of feeder cells occurred via paracrine communication between hES cells and feeder cells. Further investigation on how adipogenesis of feeder cells affects the niche of human embryonic stem cells is required.
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