Bone Marrow-derived Side Population Cells are Capable of Functional Cardiomyogenic Differentiation
Bone Marrow-derived Side Population Cells are Capable of Functional Cardiomyogenic Differentiation
- 주제(키워드) Bone Marrow , Cardiomyogenic Differentiation , Side Population Cells
- 발행기관 한국분자세포생물학회
- 발행년도 2008
- 총서유형 Journal
- UCI G704-000079.2008.25.2.022
- KCI ID ART001235403
- 본문언어 영어
초록/요약
It has been reported that bone marrow (BM)-side population (SP) cells, with hematopoietic stem cell activity, can transdifferentiate into cardiomyocytes and contribute to myocardial repair. However, this has been questioned by recent studies showing that hematopoietic stem cells (HSCs) adopt a hematopoietic cell lineage in the ischemic myocardium. The present study was designed to investigate whether BM-SP cells can in fact transdifferentiate into functional cardiomyocytes. Phenotypically, BM-SP cells were 19.59% ± 9.00 CD14+, 8.22% ± 2.72 CD34+, 92.93% ± 2.68 CD44+, 91.86% ± 4.07 CD45+, 28.48% ± 2.24 c-kit+, 71.09% ± 3.67 Sca-1+. Expression of endothelial cell markers (CD31, Flk-1, Tie-2 and VEGF-A) was higher in BMSP cells than whole BM cells. After five days of coculture with neonatal cardiomyocytes, 7.2% ± 1.2 of the BM-SP cells expressed sarcomeric α-actinin as measured by flow cytometry. Moreover, BM-SP cells co-cultured on neonatal cardiomyocytes fixed to inhibit cell fusion also expressed sarcomeric α-actinin. The co-cultured BM-SP cells showed neonatal cardiomyocyte- like action potentials of relatively long duration and shallow resting membrane potential. They also generated calcium transients with amplitude and duration similar to those of neonatal cardiomyocytes. These results show that BM-SP cells are capable of functional cardiomyogenic differentiation when cocultured with neonatal cardiomyocytes.
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