Conjugated Biomimetic Polymer Sensors Employing Fluorescence Resonance Energy Transfer
Conjugated Biomimetic Polymer Sensors Employing Fluorescence Resonance Energy Transfer
- 주제(키워드) Polydiacetylene , Fluorescence resonance energy transfer (FRET) , Inclusion complex , Biomolecular recognition
- 발행기관 한국바이오칩학회
- 발행년도 2007
- 총서유형 Journal
- UCI G704-SER000001574.2007.1.4.008
- KCI ID ART001364137
- 본문언어 영어
초록/요약
In this study, conjugated biomimetic polymer sensors composed of 10,12-Pentacosadiynoic acid (PCDA) and fluorescent dye BO558 were prepared. Polymerized PCDA vesicles show unique colorimetric and fluorescent changes by external stresses, that is, from blue to red and from non-fluorescent to red-fluorescent. However, the quantum yield of PCDA alone is very low (blue phase: 10<sup>- 4</sup>, red phase: 0.02). Such disadvantage can be overcome via the use of fluorescence resonance energy transfer (FRET) by incorporating fluorescent dye molecules into PCDA vesicles. In this article, we report that heterogeneous PCDA/BO558 vesicle systems can selectively detect the inclusion complex of α-cylcodextrin by using fluorescent spectroscopy. While the fluorescence intensity of BO558 was considerably decreased in polymerized blue-phase PCDA/BO558 vesi-cles due to energy transfer, the intensity was increased and recovered upon reaction with α-cyclodextrin (CD). On contrary, it was not changed upon exposure to γ-CD, indicating the specific detection performance of the sensor. Such fluorescent changes are considered to result from reduced energy transfer due to a conform-ational change of the conjugated PCDA polymer backbone by the inclusion compl-exation of α-CD. These FRET characteristics of PCDA/BO558 vesicles would apply to a wider range of biomolecular recognition events.
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