Characterization of a Novel Necrotic Response of Glycine max Line ‘PI96188’ toXanthomonas axonopodis pv. Glycines
Characterization of a Novel Necrotic Response of Glycine max Line ‘PI96188’ toXanthomonas axonopodis pv. Glycines
- 주제(키워드) bacterial pustule disease , defense gene , resistance , soybeanSoybean (Glycine max (L) Merr.) is one of the mostprotein-rich oilseed crops for the leading edible oil sourceworldwide (Manjaya and Pawer , 1999). Bacterial pustuledisease caused by Xanthomon
- 발행기관 한국식물병리학회
- 발행년도 2007
- 총서유형 Journal
- UCI G704-000528.2007.23.3.015
- KCI ID ART001194071
초록/요약
Typical susceptible symptoms of the bacterial pustule disease caused by Xanthomonas axonopodis pv. glycines are pustule and chlorotic haloes that usually occur in leaves of Glycine max plants. The soybean genotype ‘PI96188’ showed an atypical response to all tested races X. axonopodis pv. glycines, accompanied with necrosis without chlorotic haloes on the underside of the necrotic symptoms. X. axonopodis pv. glycines 8ra grew to levels from 10 to 100 fold lower on PI96188 than on susceptible cultivar Jinjoo1, but 10-fold higher than on the resistant cultivar CNS. The chlorophyll content in PI96188 leaves remained unchanged until 12 days after bacterial infection. Ultrastructural observation showed that the infected leaf cells of PI96188 had intact normal chloroplasts compared to those of the susceptible cultivar Jinjoo1. Chloroplast degradation or the absence of chloroplasts in cells of the infected tissues was observed in Jinjoo1. Senescence-related ACS7 gene was significantly induced in PI96188 compared to those in Jinjoo1 at 2 days after inoculation. While photosynthesisrelated rbcS gene showed the dramatic change in Jinjoo1, this gene was constitutively expressed in PI96188. However, expression of the defense-related genes, such as peroxidase and isoflavone synthase in the infected PI96188 leaves was similar to that in Jinjoo1. Together, these results suggest that the novel necrotic symptom in PI96188 is a kind of resistant response different from a typical hypersensitive response in the resistant genotypes.
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