Fabrication and Characterization of Silicon-Based Photodiode for Detection of Luminol Chemiluminescence in a Biosensor
Fabrication and Characterization of Silicon-Based Photodiode for Detection of Luminol Chemiluminescence in a Biosensor
- 주제(키워드) Luminol , Chemiluminescence , Biosensor , Photodiode
- 발행기관 한국물리학회
- 발행년도 2003
- 총서유형 Journal
- UCI G704-000411.2003.42.III.039
- KCI ID ART000990000
- 본문언어 영어
초록/요약
In this paper, a silicon-based p$^+$-n junction photodiode is fabricated by semiconductor processing as a detector for luminol chemiluminescence (CL) that is used in present the biosensor. The optimum conditions of the CL reaction are determined by horse-radish peroxidase (HRP) concentration, the reagent mixture ratio of luminol with HRP and hydrogen peroxide (H$_2$O$_2$) in the reactor well made of polydimethylsiloxane (PDMS). The output current of the photodiode is measured for CL by H$_2$O$_2$ concentration under 2 mM luminol and 25 units HRP. As the H$_2$O$_2$ concentration increases, the photodiode output current peak values increase. The photocurrent is linearly related to the H$_2$O$_2$ concentration in the range 0.01 $\sim$ 0.5 mM, and then the sensitivity of the photodiode is 23.429 pA/mM$\cdot$mm$^2$.
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