Cellular-Scale Aptamer-OLED Rods Emitting on Human Cells Expressing EpCAM Proteins
- 주제(키워드) OLED microparticles , Aptamer-hybrids , Human cell recognition , Graphene oxide
- 발행기관 고려대학교 KU-KIST융합대학원
- 지도교수 안동준
- 발행년도 2020
- 학위수여년월 2020. 2
- 학위구분 석사
- 학과 KU-KIST융합대학원 NBIT융합전공
- 원문페이지 73 p
- UCI I804:11009-000000127515
- DOI 10.23186/korea.000000127515.11009.0000944
- 본문언어 영어
- 제출원본 000046023563
초록/요약
In this thesis, we succeeded in protein and cell recognition by means of converging an OLED material and an aptamer that possesses a three-dimensional DNA structure. Union of the two generated micrometer-scale rod particles yielding luminescent intensity that enhanced upon specific recognition of protein and cell. As an emitter, chosen was tris(8-hydroxyquinoline) aluminum (Alq3), one of the most widely-used green OLED components in the display technology. Epithelial cell adhesion molecule (EpCAM) was selected as a target since its corresponding aptamer as a capture probe has been well-established in cancer studies. The fabricated aptamer-OLED microrods revealed the stark capability of discriminating human EpCAM protein over human EGFR and anti-human IgG. Human epithelial cells expressing membrane human EpCAM proteins found detectible by theses microrods while mouse rectum carcinoma cells remained invisible. As a cellular-scale OLED particle, it was easier to observe the fluorescence amplification in single particle as well as in multiparticulate solution, induced by target-probe interaction. Interestingly, the use of graphene oxide as a quencher made these aptamer-Alq3 microrods more effective to enhance the fluorescence signal to 2-fold when interacted with human oral squamous carcinoma cells. This new initiative, applying biometric functionality to the technological value of organic semiconductor materials, will provide a new platform for the evolution of OLED materials.
more목차
Abstract i
Table of Contents iii
List of Figures v
1. Introduction 1
1.1. Introduction 1
1.2. Organic Light-Emitting Molecules: Alumina Quinoline 3
1.3. Biorecognitive Materials: Aptamer 5
1.4. Quenching Materials: Graphene Oxide (GO) 7
1.5. Objective 9
2. Experimental Section 11
2.1. Materials and Instruments 11
2.2. Fabrication of Aptamer-Guided Alq3 Miroparticles 13
2.2.1. Preparation of Aptamer 13
2.2.2. Fabrication of CTAB, ssDNA and Aptamer Alq3 Rods 15
2.3. Recognition of Target Proteins and Cells by Aptamer-Alq3 MicroParticles 17
2.3.1. Preparation of Human EpCAM Proteins 17
2.3.2. Treatment of The Proteins with Alq3 Particles 18
2.3.3. Preparation of HSC-3, OMEC and CMT-93 Cells 19
2.3.4. Treatment of The Cells with Alq3 Particles 20
2.4. Recognition of Human Epithelial Cells by Graphene Oxide (GO) Based Alq3 Particles 21
2.5. Measurements 23
3. Results and Discussion 24
3.1. Optical Properties Analysis of Human EpCAM Aptamer-Alq3 24
3.1.1. Morphological Analysis of Aptamer-Alq3 Particles 24
3.1.2. Fluorescence Analysis of Aptamer-Alq3 Particles 26
3.2. Specific Response of human EpCAM Proteins by Alq3 28
3.2.1. Fluorescence Analysis of Aptamer-Alq3 Microparticles when exposed to human EpCAM protein 28
3.2.2. Fluorescence Analysis of various Alq3 Particles when exposed to different proteins 30
3.3. Human Epithelial Cellular Response of Aptamer-Alq3 34
3.3.1. Fluorescence Analysis of Aptamer-Alq3 Particles when Exposed to Human Squamous Carcinoma Cells (HSC-3) 34
3.3.2. Fluorescence Analysis of Aptamer-Alq3 Particles when Exposed to Human Oral Mucosal Cells (OMEC) 38
3.3.3. Fluorescence Analysis of Aptamer-Alq3 Particles when Exposed to Mouse Rectum Carcinoma (CMT-93) 41
3.3.4. Fluorescence Analysis of human EpCAM Aptamer-guided Alq3 with Graphene Oxide after Interaction with Cells 44
4. Conclusion 50
References 51
