Immunoassay Love Wave Biosensor for Liquid Methamphetamine Detection
- 주제(키워드) Love Wave , Immunoassay , Methamphetamine , Mass loading effect , Acousto-electric effect , Surface Acoustic Wave
- 발행기관 고려대학교 대학원
- 지도교수 주병권
- 발행년도 2020
- 학위수여년월 2020. 2
- 학위구분 석사
- 학과 대학원 전기전자공학과
- 원문페이지 79 p
- UCI I804:11009-000000127261
- DOI 10.23186/korea.000000127261.11009.0000952
- 본문언어 영어
- 제출원본 000046025864
초록/요약
Recently, many researches have been conducted to detect biomaterials using surface acoustic wave-based sensors. Love Wave sensor, a kind of surface acoustic wave sensor, makes surface acoustic wave more sensitive through guiding layer and has high sensitivity even in liquid state. The sensor is the most recently developed device designed to be integrated into a "lab-on-a-chip" system, and in recent years, research on the analysis of DNA, antibodies and proteins in solutions is also underway. The sensor also benefits from being a compact, cost-effective and portable device for point-of-care testing (POCT) that can be used at the primary care level without a laboratory infrastructure. However, most of them are detected by mass loading effects due to the immunoassay and enzymatic reactions of surface acoustic wave biomaterials. As a sensing layer for detecting them, Au, which has already developed many biomaterial immobilization techniques, is used as a material. In this case, however, the mass loading effect is limited and it is difficult to detect biomaterials with small molar concentrations and molar masses. Therefore, we used the graphene monolayer as the sensing layer to improve the sensitivity and response time for Methamphetamine by using the Acousto-Electric (AE) effect according to the change in the surface conductivity of graphene according to the immune response of biomaterials. Through this research, we can see the possibility of a new field for LW biosensor-based AE effect that can apply various 2D nanomaterials with high conductivity as sensing layer.
more목차
CHAPTER 1. INTRODUCTION 1
CHAPTER 2. THEORETICAL BACKGROUND 5
2. 1. Operation principle of SAW sensors 5
2. 2. Love Wave structure 6
2. 3. SAW sensor design 8
2. 3. 1. Piezoelectric subsstrate 8
2. 3. 2. IDT 12
2. 3. 3. Guiding layer 16
2. 3. 4. Sensing layer 18
2. 3. 5. Flow diagram of the simulation 21
2. 4 Immunoassay 23
CHAPTER 3. EXPERIMENTAL DETAILS 25
3. 1. Love Wave biosensor fabrication 25
3. 2. Love Wave biosensor detection setup 29
CHAPTER 4. RESULT AND DISCUSSION 30
4. 1. Love Wave sensor S21 charactersistics 30
4. 2. Love Wave sensor basic characteristics depends on guiding layer 33
4. 3. Love Wave sensor basic characteristics depends on sensing layer 35
4. 4. Frequency stability characteristics depends on ion concentration 37
4. 5. Biotin-Streptavidin binding characteristics 39
4. 6. Methamphetamine binding characteristics 49
CHAPTER 5. CONCLUSION 60
LIST OF REFERENCES 62
