Analysis of Protein Biomarkers in Human Serum for Major Depressive Disorder
- 주제(키워드) Biomarker , depression , proteomics , suicide
- 발행기관 고려대학교 대학원
- 지도교수 김익환
- 발행년도 2018
- 학위수여년월 2018. 2
- 학위구분 석사
- 학과 대학원 생명공학과
- 세부전공 의약나노바이오시스템
- 원문페이지 61 p
- 실제URI http://www.dcollection.net/handler/korea/000000080582
- 본문언어 영어
초록/요약
Depression is a disease with symptoms such as sadness, decreased self-esteem, sleep disturbances, loss of appetite, fatigue, poor concentration and Studies have shown that about 60% of people who commit suicide are mood disorders. Since suicide causes serious problems in individuals and society, it is necessary to have a system that can anticipate and prevent them.The goal of this study is to identify useful indicators to diagnose and predict suicidal behavior through the verification of plasma proteins and to examine the correlation with psychological assessments. We included 111 individuals with major depressive disorder from Anamkorea university hospital of Seoul. At baseline, we assessed Complement component 3 (C3), von-willebrand factor (vWF) and C-reactive protein (CRP) levelsby Enzyme-Linked Immunosorbant Assay (ELISA) and 8 kinds of psychological assessments (17 HAM-D, 21 HAM-D, STAI-S, SIS, BIS, K-STAXI, TAS-20, PSS). We performed ANCOVA analysis to identify the differences of C3 level within groups (normal, non-suicidal and suicidal patients) and adjusted for age, smoking and drinking. In addition, patients were divided into subgroups based on demographic characteristics such as age, sex, smoking and alcohol groups. We conducted correlation analysis to confirm the association with diagnostic assessments in the suicide attempted group. C3 levels higher than normal group were significantly associated with suicide attempted patients with depression (p==0.04), but not with non-suicidal patients. There are significant negative correlations between Suicide Ideation of Scale (SIS) and plasma C3 level in suicidal patients (P≤0.01). And we found significant negative association between Perceived Stress Scale (PSS) and the C3 level in non-drinking group of suicide attempted (P=0.02). Our research results suggest C3 protein can be a diagnostic marker of suicide behavior and depressive symptoms.
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Contents
Abstract
INTRODUCTION
MATERIALS AND METHODS
1. Subjects
2. Methods
2.1. Psychological assessments
2.1.1. Hamilton Depression Rating Scale (HAM-D)
2.1.2. State-Trait Anxiety Inventory (STAI-S)
2.1.3. Suicide Intent Scale (SIS)
2.1.4. Barratt Impulsiveness Scale (BIS)
2.1.5. K-State Trait Anger Expression Inventory (K-STAXI)
2.1.6. Toronto Alexithymia Scale-20 (TAS-20)
2.1.7. Perceived Stress Scale (PSS)
2.2. Biological assessment
2.3. Statistical analysis
RESULTS
1. Verification of protein levels
1.1. Comparison of plasma protein levels among the normal group, non-suicidal and suicidal patients
1.2. Comparison of plasma C3 protein level among the groups
1.3. Logistic regression analysis of C3 protein for diagnostic marker
1.4. Comparison of C3 protein as distinguishing model in MDD patients and suicidal patients
2. Correlation between C3 level and psychological assessments
2.1. 17 HAM-D
2.2. 21 HAM-D
2.3. STAI-S
2.4. SIS
2.5. BIS
2.6. K-STAXI
2.7. TAS-20
2.8. PSS
DISCUSSION
REFERENCES
ABSTRACT IN KOREAN
