Early-life exposure to antidepressant changes stress-induced behavior in the adulthood
- 주제(키워드) depression , behavior , stress
- 발행기관 고려대학교 대학원
- 지도교수 윤봉준
- 발행년도 2017
- 학위수여년월 2017. 8
- 학위구분 석사
- 학과 대학원 생명과학과
- 원문페이지 68 p
- 실제URI http://www.dcollection.net/handler/korea/000000077315
- 본문언어 영어
- 제출원본 000045915296
초록/요약
Early life exposure to antidepressants has a correlation between the development of behavioral and neuroendocrine abnormalities later in life. Animal models in previous studies have proved that early exposure to some antidepressants can alter behaviors in adulthood. In this study, I injected clomipramine (CLI), a serotonin and norepinephrine reuptake inhibitor, to neonatal mice for several days and conducted three chamber social interaction test to verify the depression-related behavioral alteration in 8 weeks. Three chamber social interaction test estimates sociability and interest in social novelty in rodent models of CNS disorders. In previous studies, genes identified differentially expressed in the dorsal raphe nucleus (DR), which has long been implicated in depression, of these mice using microarray analysis of gene expression. Also, it turned out growth hormone (Gh) gene expression was significantly downregulated in clomipramine-treated mice as well as in mice that experienced social defeat stress. Gh, encoding a peptide hormone, is known to play a role in stress response by raising blood glucose concentration in addition to roles in growth, cell reproduction, and cell regeneration. I’m trying to investigate the functional roles of Gh in DR in depression-related behaviors using AAV-mediated gene delivery approach.
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Table of Contents
List of Figures - iii
Abstract - iv
1. Introduction - 1
1.1. Depressive disorder and early life stress - 1
1.2. The monoamine hypothesis of depression - 3
1.3. Dorsal raphe nucleus (DRN) and depression 4
1.4. Animal model of depression 6
1.5. Growth hormone (Gh) in depression - 8
1.6. Sociable behaviors in depression - 10
2. Materials and Methods - 11
2.1. Animals and drug treatment - 11
2.2. Social defeat (SD) stress - 11
2.3. Three chamber social interaction test 13
2.4. Virus construct and preparation 15
2.5. Stereotaxic surgery 15
3. Results
3.1. The effect of administration of clomipramine in early life in weight changes - 17
3.2. Neonatal administration of clomipramine has an effect on the stress response in social interaction behavior 18
3.3. Administration of clomipramine and social defeat stress affect mices behaviors 19
3.4. Over expression of growth hormone in dorsal raphe nucleus - 20
3.5. The effect of Gh injection in SAL and CLI treated mice in session 1 and 2 - 21
4. Discussion 43
5. References 47
Abstract in Korean - 59
