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Study on the novel processing methods for the production of Panax ginseng with improved ginsenoside profile

초록/요약

The effects of various processing methods including steaming, fermenting, enzyme reaction and high-pressure reaction for ginseng were estimated for ginsenosides profiles. Ginsenosides of ginseng collected from cultivation location such as Ganghwa (4.12±0.07 mg/g), Nonsan (2.63±0.01 mg/g), Boeun (3.82±0.08 mg/g), Anseong (3.91±0.02 mg/g), Icheon (2.78±0.02 mg/g) and Hongcheon (2.56±0.06 mg/g) which are the main cultivation locations in South Korea were analyzed, and then Ganghwa ginseng was selected as the sample. The selected ginseng samples were processed by the above four processing methods; steaming, fermenting (Bacillussubtilis natto, Lactobacillus plantarum and Phellinus linteus), enzymaic reaction (Plantase TL, Rohament CL, Peclyve LI, Viscozyme L, Ultraflo Max and Sumizyme AC) and high-pressure reaction. Among them, only steaming was used for processing of ginseng roots. Fermentation, enzyme reaction and high-pressure reaction were used for ginseng extract. The 15 major ginsenosides such as Rb1, Rb2, Re, Rd, Re, Rf, Rg1, Rg2, Rg3, Rg5, Rh1, Rh2, Rk1, F2 and compound K (Cpk) of each processed ginseng samples were analyzed by HPLC. The steaming, fermentation, enzyme treatment, and high-pressure treatment had various ginsenoside profiles. Since the total amount of ginsenosides or the ginsenoside profiles varies with each processing method, it is necessary to select the appropriate processing method according to the target ginsenoside. This thesis includes the results of comparison of the changes in ginsenosides obtained from different treatment such as steaming, fermentation, enzyme treatment, and high-pressure treatments and the results to propose optimal processing methods for desired ginsenosides production.

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목차

1. Introduction 1
2. Materials 6
2-1. Saponin analysis 6
2-2. Processing of ginsenosides to transformation of ginsenoside profiles 7
2-2.1. Steaming Processing 7
2-2.2. Fermentation Processing 8
2-2.3. Enzyme Treatment Processing 8
2-2.4. High Pressure Treatment Processing 9
3. Results and Discussion 21
3-1. The effect of cultivation location and drying on the profile of ginsenosides 21
3-2. The effect of steaming processing on the profile of ginsenosides 22
3-3. The effect of fermentation processing on the profile of ginsenosides 22
3-4. The effect of enzyme treatment processing on the profile of ginsenosides 23
3-5. The effect of high-pressure treatment processing on the profile of ginsenosides 24
4. Conclusion 26
5. References 27
6. Korean abstract 33

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