Separation of the sub-micro particles using standing surface acoustic wave
- 주제(키워드) sub-micro particle , Standing Surface Acoustic Wave , separation
- 발행기관 고려대학교 대학원
- 지도교수 신세현
- 발행년도 2017
- 학위수여년월 2017. 2
- 학위구분 석사
- 학과 대학원 기계공학과
- 원문페이지 64 p
- 실제URI http://www.dcollection.net/handler/korea/000000073021
- 본문언어 영어
- 제출원본 000045897332
초록/요약
Separation of small sized-biomolecules such as virus, bacteria and exosomes rises as a significant technique especially in laboratory analysis and clinical diagnosis. In our previous research, we separated micro particles using a standing surface acoustic wave (SSAW). However, we also found that acoustic force exerted on sub-micro particles was too small to separate them within a short distance. In this research, we suggest the advanced research for separating smaller particles below micro scale by optimizing microfluidic channel design and enhancing the power of acoustic radiation force using impinging jet cooling system. Due to our advanced separating system, the microfluidic chip could endure the high voltage so that we could control the migration of sub-micro particles. Under this system, we confirmed the movement of particles with diameter of 200 nm and 500 nm at the same voltage and the distance of toward wall from the center line of channel was different due to acoustic radiation force differences. In addition, we separated sub-micro particles with diameter of 100 nm, 200 nm and 500 nm particles. In conclusion, we successfully separated sub-micro particles by size and confirmed the possibility in separation of nano-size particles which is a crucial improvement from previous researches.
more목차
1. Introduction .................................................................................................1
1.1 Importance of biomolecules separation............................................. 1
1.2 Previous separation techniques …......................................................4
1.3 Objectives of this study...................................................................... 9
2. Material and Method ................................................................................11
2.1 Manufacturing device...................................................................... 11
2.2 Separation system setup .................................................................. 14
2.3 Preparation of samples and channel coating.................................... 17
2.4 Impinging jet cooling system.......................................................... 19
2.5 Mechanisms .................................................................................... 21
3. Results and Discussion................................................................................. 23
3.1 Verification of cooling system......................................................... 23
3.2 Migration of 500 nm and 200 nm particles……………..…………27
3.3 Separation of 500 nm and 200 nm particles.................................... 30
3.4 The efficiency of sub-micro particle separation.............................. 37
4. Conclusions................................................................................................... 44
5. References .................................................................................................... 46
