Physiological interaction between copper and zinc in Aspergillus fumigatus
- 주제(키워드) aspergillus fumigatus , copper and zinc
- 발행기관 고려대학교 대학원
- 지도교수 윤철원
- 발행년도 2017
- 학위수여년월 2017. 2
- 학위구분 석사
- 학과 대학원 생명과학과
- 세부전공 세포생물학 전공
- 원문페이지 48 p
- 실제URI http://www.dcollection.net/handler/korea/000000071569
- 본문언어 영어
- 제출원본 000045897453
초록/요약
Abstract It has been reported that copper and zinc are virulence factors in pathogenesis of Aspergillus fumigatus. Mac1 is well-known transcription factor in copper homeostasis of Saccharomyces cerevisiae and mrpA of A. fumigatus is a homologue of Mac1. We investigated the function of mrpA on zinc metabolism to figure out how mrpA associated with metal homeostasis. mrpA deletion strain exhibited growth defect on Aspergillus minimal medium (AMM) and complex medium (CM) both. Interestingly, zinc limitation suppressed growth defect of mrpA deletion strain. And expression of the genes encode zinc transporters significantly reduced compared with wild type when mrpA was deleted. In addition, mrpA deletion strain showed growth defect to the cell wall stress-inducing agents. Superoxide dismutase (SOD) activity was reduced when the mrpA was deleted and mrpA deletion strain was sensitive to H2O2. Taken these results together, we suggest that MrpA monitors the environmental change of zinc concentration as concerted manner with copper homeostasis in A. fumigatus.
more목차
Contents
List of Tables…………………………………………………..…. Ⅲ
List of Figures……………………………………………………. Ⅲ
Abstract…………………………………………………………… 1
Introduction…………………………………..…………….…….. 2
Materials and Methods
Strains, media and culture conditions……………………………. 6
plasmid construction……………………………………………... 6
Inductively coupled plasma/optical emission spectrometry……... 7
Plate assays……………………………………………………..... 8
Northern blot analysis……………………………………………. 8
Microarrays……………………………………………………..... 9
Superoxide dismutase (SOD) zymogram……………………....... 9
Results
MrpA controls the expression level of zinc transporters in A. fumigatus……………………………………………………….. 12
MrpA is physiologically regulated by zinc concentration……… 16
H2O2 sensitivity and Low SOD activity of mrpA gene deletion mutant………………………………………………………….. 23
MrpA deletion strain showed growth defect to the cell wall stress-inducing agents………………………………………………… 27
Discussion……………………………………………………….. 30
References………………………………………………………. 33
Abstract in Korean……………………………………………... 38
List of Tables
Table 1. Strain used in this study…………………………………. 11
Table 2. Names and sequences of the primers used in the present study………………………………………………………………. 11
List of Figures
Figure 1. MrpA regulates the expression of zinc transporters of Aspergillus fumigatus……………………………………………... 14
Figure 2. Growth assay of mrpA deletion strain and regulation of mrpA gene expression by zinc utilization…………………………. 18
Figure 3. Growth assay of mrpA deletion strain in copper, zinc, iron and cellular copper content………………………………………... 20
Figure 4. H2O2 sensitivity and low SOD activity of mrpA gene deletion mutant……………………………………………………. 24
Figure 5. Plate assay of mrpA deletion strain in cell wall stress agents test………………………………………………………………… 28
