3D Brain Model Construction by a Simultaneous Control of Cell-Cell and Cell-Surface Interactions
- 주제(키워드) 3D Brain Model
- 발행기관 고려대학교 대학원
- 지도교수 이상훈
- 발행년도 2016
- 학위수여년월 2016. 8
- 학위구분 석사
- 학과 대학원 바이오융합공학과
- 세부전공 바이오융합공학전공
- 원문페이지 50 p
- 실제URI http://www.dcollection.net/handler/korea/000000069455
- 본문언어 영어
- 제출원본 000045884555
초록/요약
Objective. A three-dimensional (3D) neural tissue model that involves cell-cell and cell-ECM interactions is important for the comprehensive understanding of neural development and neurological diseases, including axonopathy. Approach. Here, a newly developed microplatform for the simultaneous control of neuronal cell-cell and cell-extracellular matrix (ECM) interactions is reported. Concave microwells, extensively used for the autonomous cell aggregation, are prepared in order to generate 3D cell-cell interactions. Additionally, the surface of concave wells is functionalized with (3-aminopropyl)triethoxysilane (APTES), carbon nanotube (CNT), poly-L-lysine (PLL), and laminin, for the control of neuronal cell-ECM interactions. Significance. Using this platform, the effects of neurite outgrowth based on the cell-ECM and cell-cell interactions are simultaneously investigated in 3D environment. Furthermore, amyloid-β (Aβ)-induced axonopathy, representing a pathological feature of neurodegenerative diseases in vivo, is also examined. Aβ treatment leads to a significant degeneration of neurite outgrowth and neuronal morphology. The proposed neural tissue model allows future studies of various neurodegenerative diseases.
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ABSTRACT
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
2. 1. Surface-modification process
2. 2. Surface confirmation
2. 3. Isolation and seeding of prenatal rat cortical neurons
2. 4. Cell adhesion assay
2. 5. Aβ-induced neurotoxicity assay
2. 6. SEM analysis
2. 7. Fixation and immunocytochemistry
2. 8. Statistical Analysis
Ⅲ. RESULTS
3. 1. Characterization of Surface-Treated Polydimethylsiloxane (PDMS) Microwells
3. 2. Atomic Force Microscopy (AFM) Analysis
3. 3. Attenuated Total Reflectance Fourier Transform (ATR FT)-IR Spectroscopy
3. 4. Cell adhesion assay
3. 5. Cell Aggregation
3. 6. Neurospheroid and Neurite Outgrowth Formation in PDMS Microwells
3. 7. Aβ-induced Morphological Changes of the Neurospheroids
Ⅳ. DISCUSSION
Ⅴ. CONCLUSIONS
Ⅵ. REFERENCES
