Effect of Low Level Light Therapy on Macrophage Conditioned Medium-Stimulated Annulus Fibrosus Cells
대식세포 유래 인자 매개 염증유도 섬유륜 세포에서의 저출력레이저 자극 효과
- 주제(키워드) Inflammation , Intervertebral disc , Annulus Fibrosus , Low Level Light Therapy
- 발행기관 고려대학교 대학원
- 지도교수 최혁
- 발행년도 2016
- 학위수여년월 2016. 2
- 학위구분 석사
- 학과 대학원 의과학과
- 세부전공 의공학전공
- 원문페이지 46 p
- 실제URI http://www.dcollection.net/handler/korea/000000064797
- 본문언어 영어
- 제출원본 000045882510
초록/요약
Intervertebral disc degeneration (IVD) is one of the important causes of low back pain and is associated with inflammation induced by interaction between macrophages and the human annulus fibrosus (AF) cells. Low level light therapy (LLLT) has been widely known to regulate inflammatory reaction. However, the effect of LLLT on macrophage-mediated inflammation in the AF cells has not been studied till date. The aim of this study is to mimic the inflammatory microenvironment and to investigate the anti-inflammatory effect of LLLT at a range of wavelengths (405, 532 and 650 nm) on the AF treated with macrophage-like THP-1 cells conditioned medium (MCM) containing pro-inflammatory cytokines and chemokines (interleukin-1beta, tumor necrosis factor-alpha, interleukin-6 and 8). We observed that AF cells exposed to MCM secrete significantly higher concentrations of IL-6, IL-8, IL-1β and TNF-α. LLLT markedly inhibited secretion of IL-6 at 405 nm in a time-dependent manner. Level of IL-8 was significantly decreased at all wavelengths in a time-dependent manner. We showed that MCM can induce the inflammatory microenvironment in AF cells and LLLT selectively suppressed IL-6 and 8 levels. The results indicate that LLLT is a potential method of IVD treatment and provide insights into further investigation of its anti-inflammation effect on IVD.
more목차
ABSTRACT i
Table of Contents iii
List of Figures v
List of Tables vi
Introduction 1
1.1 Research Background 1
1.2 Purpose of Research 2
Materials and Methods 5
2.1 AF cells isolation and culture 5
2.2 Activation of macrophage-like THP-1 cells and MCM (macrophage conditioned medium) collection 6
2.3 Stimulation of AF cells with MCM 7
2.4 Low Level Light Therpy (LLLT) 7
2.5 Biochemical analysis, enzyme-linked immunosorbent assay (ELISA) and lactate dehydrogenase (LDH) release measurement 9
2.6 Statistical analysis 10
Results 12
3.1 Characteristics of activated macrophage-like THP-1 cells 12
3.2 Time course of inflammatory mediator production by MCM stimulation in AF cells 14
3.3 LLLT decreases secretion of inflammatory mediators by MCM-stimulated AF cells 16
3.4 LDH Release by MCM-stimulated AF cells with LLLT 19
Discussion and conclusions 21
References 26
초록 (국문) 34
