Lysing Model Vesicles with Surfactant Vehicles in Encapsulated Particles
- 주제(키워드) surfactant
- 발행기관 고려대학교 KU-KIST융합대학원
- 지도교수 안동준
- 발행년도 2016
- 학위수여년월 2016. 2
- 학위구분 석사
- 학과 KU-KIST융합대학원 NBIT전공
- 원문페이지 84 p
- 실제URI http://www.dcollection.net/handler/korea/000000064745
- 본문언어 영어
- 제출원본 000045866672
초록/요약
Extracellular vesicles can transfer signals between tumor cells, and thus plays important roles in tumor development, even leading to metastasis. To inhibit the deleterious effects of extracellular vesicles, we explored the possibility of targeting and lysing these vesicles using surfactants. Quantitative analysis was carried out for the lysing efficacy of surfactants and the mode of delivery for these surfactants was investigated. This study aimed to develop potential therapeutic agent that can target and lyse nanometer-sized extracellular vesicles. Novel characteristics of the surfactant such as versatile structures and phases, depending on the concentration and temperature, enabled us to discover a novel therapeutic agent.
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Contents
Chapter 1. Introduction 12
1. 1. Functions and roles of extracellular vesicles from cells 12
1. 2. Literature reviews of surfactants lysing cells 15
1. 3. Objective 16
Chapter 2. Experimental 17
2. 1. Materials 17
2. 2. Experimental methods 19
2.2.1. Fabrication of NBD-PE labelled DOPC vesicles 19
2.2.2 Encapsulation of CTAB surfactant vehicles 19
2.3. Analytical methods 21
Chapter 3. Results and Discussion 22
3. 1. Lysing NBD-PE labelled DOPC vesicles with surfactant micelles 22
3.1.1. Population analysis of NanoSight results 22
3.1.2. Population analysis of FACSCalibur results 29
3.1.3. Surface charge analysis of Zeta potential results 35
3.1.4. Images of the vesicles by Confocal laser scanning microscopy and CryoTEM 39
3. 2. Encapsulation of CTAB surfactant vehicles 47
3.2.1. Principle of Kraffts temperature of surfactant 47
3.2.2. Surface charge analysis of Zeta potential results 48
3.2.3. Chemical structure analysis of FT-IR 49
3.2.4. Separation of smaller surfactant particles 54
3.2.5. Observation of surfactant particles regarding temperature change 57
3.2.6. Release of CTAB surfactant from surfactant particles with LC-MS analysis 62
3.3 Lysing NBD-PE labeled DOPC vesicles with surfactant particles 66
3.3.1. Population analysis of FACSCalibur results 66
Chapter 4. Conclusions 68
References 69
Supplementary 73
Acknowledgements 84
