Effect of Panax ginseng Extract on Human Dermal Fibroblast Proliferation and Collagen Synthesis
인삼 추출물이 진피섬유모세포의 증식과 교원질합성에 미치는 영향
- 주제(키워드) ginseng , Panax ginseng , wound healing , fibroblast
- 발행기관 고려대학교 대학원
- 지도교수 한승규
- 발행년도 2015
- 학위수여년월 2015. 8
- 학위구분 석사
- 학과 대학원 의학과
- 세부전공 성형외과학 전공
- 원문페이지 25 p
- 실제URI http://www.dcollection.net/handler/korea/000000060555
- 본문언어 영어
- 제출원본 000045851124
초록/요약
ABSTRACT Effect of Panax ginseng Extract on Human Dermal Fibroblast Proliferation and Collagen Synthesis Jinying LI Major in Plastic Surgery, Department of Medical science, Graduate School of Korea University, Seoul, Korea (Director: Professor Seung Kyu Han, M.D., Ph.D.) Purpose: Panax ginseng (or Korean ginseng) has been used traditionally for a wide range of preventative and therapeutic purposes, including increasing energy, vitality, and for anti-aging. Current studies have demonstrated that P. ginseng has various biological activities, including angiogenesis, immuno-stimulation, anti-microbial, and anti-inflammatory actions. Therefore, we hypothesized that P. ginseng may also play an important role in wound healing. However, few studies have been conducted on the wound healing effects of P. ginseng. Thus, the purpose of this in vitro pilot study was to determine the effect of P. ginseng on activities of fibroblasts, which are key wound-healing cells. Methods: Cultured human dermal fibroblasts were treated with one of six concentrations of P. ginseng: 0, 1, 10, and 100 ng/ml and 1 and 10 µg/ml. Cell proliferation was determined 3 days post-treatment using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay, and collagen synthesis was evaluated by the collagen type I carboxy-terminal propeptide method. Cell proliferation levels and collagen synthesis were compared among the groups. Results: The 10 ng/ml to 1 µg/ml P. ginseng treatments significantly increased cell proliferation, and the 1 ng/ml to 1 µg/ml concentrations significantly increased collagen synthesis. The maximum effects for both parameters were observed at 10 ng/ml. Conclusion: P. ginseng stimulated human dermal fibroblast proliferation and collagen synthesis at an optimal concentration of 10 ng/ml.
more목차
ABSTRACT ⅰ
Ⅰ. INTRODUCTION 1
Ⅱ. METHODS 3
Ⅲ. RESULTS 6
Ⅳ. DISCUSSION 7
Ⅴ. CONCLUSION 11
Ⅵ. REFERENCES 12
Ⅶ. TABLES and FIGURES 14
