UV-Dependent Synthesis of Silver Nanoparticles Using Fruit Extracts
- 주제(키워드) Silver nanoparticle
- 발행기관 고려대학교 대학원
- 지도교수 김성욱
- 발행년도 2015
- 학위수여년월 2015. 2
- 학위구분 석사
- 학과 일반대학원 의생명융합과학과
- 원문페이지 46 p
- 실제URI http://www.dcollection.net/handler/korea/000000057281
- 본문언어 영어
- 제출원본 000045854094
초록/요약
ABSTRACT The objective of this study was an UV-dependent Green synthesis of silver nanoparticles (AgNPs) using Malus pumila miller (Apple) and Lycopersicon esculentum (Tomato) extracts. Malus pumila miller and Lycopersicon esculentum were ground by mixer and then the juicy products were filtered through filter paper at first. Finally, the filtered extracts were filtered again for preventing bacterial contamination by 0.8㎛ syringe filter and stored in refrigerator at 4℃. Silver nitrate solution (1mM) was mixed with filtered fruit extracts and the mixture was illuminated to Ultra Violet light (312nm) for the synthesis of AgNPs. After the color of mixture change to dark-yellow or reddish- brown, the synthesis of silver nanoparticles was confirmed by Surface Plasmon Resonance (SPR) using UV-visible spectrophotometer. Fourier transformed infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM) revealed the characterization of synthesized AgNPs. The antibacterial property of AgNPs were investigated by well diffusion methods and growth curve against Stapylococcus aureus, Bacillus subtilis, Streptococcus pyogens, Escherichia coli, Pseudomonas aeruginosa. Keywords: green synthesis, silver nanoparticle, UV, antibacterial
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ABSTRACT.................................................ⅰ
CONTENTS.................................................ⅲ
LIST OF FIGURE AND TABLE..............................ⅴ
1. INTRODUCTION........................................1
2. MATERIAL AND METHODS
2.1. Chemicals and fruits collection...................6
2.2. Preparation of fruit extract......................6
2.3. UV-dependent synthesis of silver nanoparticles....7
2.4. Characterization of silver nanoparticles..........7
2.5. Antibacterial activity of silver nanoparticles
2.5.1. Agar well diffusion method......................8
2.5.2. Microbial growth in the presence of AgNPs.......9
3. RESULTS AND DISCUSSION
3.1. Color change and UV-vis spectrophotometer........10
3.2. Fortier transform-infrared spectroscopy (FT-IR)....13
3.3. Scanning electron microscopy (SEM) image of AgNPs17
3.4. Antibacterial activity of AgNPs
3.4.1. Inhibition zone in agar plate..................19
3.4.2. Growth curve of bacteria.......................22
4. CONCLUSION.........................................25
5. REFERENCES.........................................26
국문요약..............................................36
