Hydrothermal synthesis of ZnO nanowire arrays for gene delivery
- 주제(키워드) ZnO nanowire array , gene delivery
- 발행기관 고려대학교 대학원
- 지도교수 김영근
- 발행년도 2015
- 학위수여년월 2015. 2
- 학위구분 석사
- 학과 일반대학원 신소재공학과
- 세부전공 신소재공학전공
- 원문페이지 61 p
- 실제URI http://www.dcollection.net/handler/korea/000000056867
- 본문언어 영어
- 제출원본 000045827923
초록/요약
The synthesis, microstructural analysis, and surface functionalization method of ZnO nanowire arrays for gene delivery are reported in this thesis. Three types of samples including ZnO nanowire arrays on Al-doped ZnO layers, ZnO layers, and ZnO nanoparticles as seed layers were prepared by a hydrothermal method. The morphology and microstructure of the ZnO nanowire arrays were analyzed by field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD). The characterization indicates that the ZnO nanowire arrays with wurtzite crystal structure were successfully produced, which has the diameter ranging from 100 to 200 nm while the length ranging from 0.5 to 2.5 um. The surface modification of ZnO nanowires were conducted by biomolecules including ZnO binding peptide (ZBP), streptavidin, biotin, and green fluorescence protein (GFP) DNA. The surface properties were investigated by a tetrazolium dye (MTT) based assay, fluorescence microscopy, and gel electrophoresis mobility shift assay (EMSA). The results appeared that ZnO nanowire arrays are suitable to gene delivery and surface modification of ZnO nanowires are successfully carried out. The gene delivery was performed to human embryonic kidney cells using ZnO nanowire arrays modified by GFP DNA. The qualitative resultant efficiency of gene delivery is influenced by the density of nanowires, due to the effective surface areas of ZnO nanowires.
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Contents
Chapter 1. Introduction .............................................................................1
Chapter 2. Background ..........................................................................4
2.1. About ZnO nanowire........................................................................4
2.2. Hydrothermal method for ZnO nanowire arrays.............................7
2.3. Nanomaterial based gene delivery method....................................10
Chapter 3. Experimental Procedure ............................................................15
3.1. Preparation of the ZnO seed layers............................................15
3.2. Synthesis ZnO nanowire arrays............................................16
3.3. Surface modification of ZnO nanowires.........................................19
3.4. Gene delivery to cell using surface modified ZnO nanowire
arrays............................................................................... 21
Chapter 4. Results and discussion ...........................................................22
4.1. Morphological and structural analyses of ZnO nanowire
arrays...................................................................................22
4.2. Surface property analysis of ZnO nanowire arrays..............28
4.2.1. Cell toxicity assay of ZnO nanowire arrays..........................28
4.2.2. Verification of surface modified ZnO nanowire............30
4.3. Gene delivery property analysis................................................34
Chapter 5. Conclusions ............................................................................40
References .................................................................................................42
