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A dip-stick biosensor using bioluminescent bacteria encapsulated in color-coded alginate microbeads for detection of water toxicity

초록/요약

The use of genetically engineered bioluminescent bacteria, in which bioluminescence is induced by different modes of toxic action, represents an alternative to acute toxicity test using living aquatic organisms (plants, vertebrates, or invertebrates) in water environment. A number of these bacterial strains have been developed, but there have been no attempts to develop hand-held type of biosensor for monitoring or identification of toxicity. I report a facile dip-stick biosensor using genetically engineered bioluminescent bacteria as a new platform for classification and identification of toxicity in water environment. This dip-stick biosensor is composed of 8 different optically color-coded functional alginate beads which encapsulate different bioluminescent bacterial strain and its corresponding fluorescent microbead together. These color-coded microbeads exhibit improved cell-stability when compared with liquid culture and easy identification since each microbead has different color code, depending on the bioluminescent bacterial strain contained. Therefore, this dip-stick biosensor can discriminate different mode of toxic actions, such as DNA damage, oxidative damage, cell-membrane damage, or protein damage in sample water. It was found that each color-coded microbead emitted distinct bioluminescence and each dip-stick biosensor showed different bioluminescence patterns, depending on the chemicals contained in LB medium, tap water, or river water samples within 2 hours. This simple and fast, but not tedious and complicated dip-stick toxicity biosensor can be used as in situ on site primary toxicity test kit for drinking water.

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목차

Table of Contents…..…………………………………………i
List of Tables………………………………………………….ii
List of Figures………………………………………………….iii
List of Abbreviations………………………………………….v
Abstract………………………………………………………..vi

1. Introduction…………………………………………………..1
2. Materials and Methods………………………………………5
3. Results and Discussion………………….………………….12
4. Conclusion…………………………………………………..24
5. Reference……………………………………………………25

Abstract in Korean……………………………………………27
Acknowledgement……………………………………………..28

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