Human Papillomavirus infection in the South Korean women
한국 여성에서 인유두종 바이러스 감염유형 분석
- 주제(키워드) HPV infection , high-risk HPV , low-risk HPV , HPV multi-infection , DNA chip , cervical cancer , precursor lesions , cervical squamous cell
- 발행기관 고려대학교 대학원
- 지도교수 설동근
- 발행년도 2014
- 학위수여년월 2014. 2
- 학위구분 석사
- 학과 일반대학원 의과학과
- 세부전공 의생명과학전공
- 원문페이지 55 p
- 실제URI http://www.dcollection.net/handler/korea/000000049109
- 본문언어 영어
- 제출원본 000045795151
초록/요약
Since the year of 2001, over 35896 tissue samples were collected from hospitals throughout South Korea. In this study, we investigated the prevalence and distribution of HPV infections in South Korean women. The collected samples were tested for HPV infection (16 genotypes of high-risk HPV and 8 genotypes of low-risk HPV) using an HPV DNA diagnostic kit ( MyGene Co. Seoul, Korea). The prevalence of HPV was 24.1% for all types combined, 12.9% for high-risk, 3.1% for low-risk, 3.9% for multi-infection, and 4.2% for unknown-risk types. In recent years, the major high-risk HPV genotypes were numbers16, 58, 53, 52, and 18 in South Korean women and their prevalence in total HPV infection were 19.30 %, 11.25 %, 8.35 %, 7.95 %, and 7.65%, respectively. The major low-risk HPV genotypes were 70, 54, 6, and 11 in South Korean women and their prevalence in total HPV infection were 7.85 %, 4.00 %, 3.00 % and 1.70 %, respectively. An epidemiological study of HPV infections in South Korean female population revealed a distinct pattern of infection. Twenty-four different genotypes were evaluated; of which genotypes number 16 and 58 were the major high-risk HPV genotypes. In addition, a few rare genotypes (number 53 and 52) were shown to be prevalent in South Korea, which suggests that the future targets for HPV protection and management in South Korea are different than those of the other countries. Further preventive and therapeutic steps to manage HPV infections in South Korea should consider the changing HPV environment.
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Contents
I. Introduction ................................................................................ 1
II. Materials and Method ............................................................................... 3
A. Subjects................................................................................... 3
B. HPV DNA Extraction…................................................................................... 3
C. PCR amplification................................................................................. 4
D. First PCR................................................................................................. 5
E. Second PCR................................................................................................. 5
F. DNA chip................................................................................................... 6
G. Hybridization................................................................ 8
H. Chip Scanning..................................................................................... 8
I. Statistical Analysis......................................................................................13
III. Result ...................................................................................14
A. Characteristics of Subjects .......................................................................... 14
B. HPV infection in South Korean women by year.................................. 16
C. HPV infection in South Korean women by Age................................... 20
D. Multiple HPV infections............................................................................ 22
E. Geographical prevalence of HPV infection in South Korea....................... 26
IV. Discussion .................................................................................... 28
V. References .................................................................................... 36
국문 개요 ........................................................................................ 43
