Stimulation of Osteoblast-like cells within Alendronate-coated Biphasic Calcium Phosphate (BCP) Scaffold
알렌드로네이트가 코팅된 이상인산칼슘 지지체에서 조골세포 자극
- 주제(키워드) Biphasic Calcium Phosphate (BCP) , Alendronate , Osteogenic differentiation , Bone tissue engineering
- 발행기관 고려대학교 대학원
- 지도교수 송해룡
- 발행년도 2013
- 학위수여년월 2013. 8
- 학위구분 석사
- 학과 일반대학원 의학과
- 세부전공 의용생체공학
- 원문페이지 33 p
- 실제URI http://www.dcollection.net/handler/korea/000000045462
- 본문언어 영어
- 제출원본 000045761804
초록/요약
Biphasic calcium phosphate (BCP) scaffolds have been widely used in the orthopedic and dental fields as osteocondutive bone substitutes. However, these scaffolds can’t stimulate osteoblast proliferation and osteogenic differentiation. The objective of this study was to investigate the alendronate (Aln)-coated BCP scaffolds stimulate more osteogenic differentiation than BCP scaffolds. Characterizations of BCP and BCP scaffolds containing Aln were confirmed with field-emission scanning electron microscopy (FE-SEM) and an attenuated total fourier-transform infrared spectroscopy (ATR-FTIR). The release kinetic of Aln from Aln(0.1 mg)/BCP and Aln(1 mg)/BCP scaffolds was evaluated for 28 days. Osteoblast differentiation of MG-63 cells on all BCP scaffolds were investigated by alkaline phosphate (ALP) activity, calcium deposition, and gene expressions. The results of FE-SEM and ATR-FTIR showed that Aln was successfully coated on BCP scaffolds. Coated Aln was released from Aln(0.1 mg)/BCP and Aln(1 mg)/BCP scaffolds in a sustained manner. In vitro results revealed that MG-63 cells grown on BCP scaffolds containing Aln increased ALP activity, calcium deposition, and up-regulation of Runx2, ALP, OCN, and OPN genes as compared with those grown on BCP scaffolds. Therefore, this study suggests that BCP scaffolds containing Aln have potential to effectively stimulate osteogenic differentiation.
more목차
Abstract
Ⅰ. Introduction 1
Ⅱ. Materials and Methods 3
Ⅲ. Results 8
Ⅳ. Discussion 11
Ⅴ. Conclusion 14
References 15
Tables 20
Legends 21
