Low power preconcentration platform utilizing Ion Concentration Polarization for the preconcentration of Influenza virus
- 주제(키워드) preconcentration , microfluidics , influenza virus , Ion Concentration Polarization (ICP)
- 발행기관 고려대학교 대학원
- 지도교수 정석
- 발행년도 2013
- 학위수여년월 2013. 2
- 학위구분 석사
- 학과 일반대학원 기계공학과
- 세부전공 기계공학전공
- 원문페이지 38 p
- 실제URI http://www.dcollection.net/handler/korea/000000039240
- 본문언어 영어
- 제출원본 000045746169
초록/요약
Analyte preconcentration has merged as a topic of interest in many laboratories over the past decades. It serves as a part of preparation procedure before analyses are conducted in either laboratories or for clinical usage. Various preconcentration platforms utilizing microfluidic technologies have been reported over the years. Low abundance analytes have been the most challenging problems in immunoassays as it is hard to detect and thus lower the sensitivity of immunoassays. With the combination of PDMS-glass substrate and also PDMS-PDMS substrate, a preconcentration platform utilizing ion concentration polarization (ICP) was developed. The efficiency of sample accumulation could be controlled by the dimension of the Nafion membrane, applied voltage as well as the temperature of the analytes. As a result, it was possible to achieve a preconcentration up to 100 folds within 5 minutes. In order for the actual concentrated analytes to be coupled to other analytical tools such as rT-PCR (real time polymerase chain reaction) and immunoassays, a certain volume is needed and the concentrated analytes have to be able to be collected but not merely for the use of in-situ detection. The preconcentrator is thus arrayed in parallel with eight channels combined. These collectable channels are arrayed in the hope to achieve a higher volume in the concentrated reservoir.
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ABSTRACT i
Table of Contents
List of Figures
List of Tables
Introduction
1.1 Research Background
1.2 Microfluidic approaches in preconcentration
1.3 Purpose of Research
Materials and Methods
2.1 Device design and fabrication
2.2 Experiment setup and sample preparation
Results and Discussions
3.1 Lower power preconcentration module
3.2 Effect of analyte temperature on preconcentration efficiency
3.3 Preconcentration of influenza virus
3.4 Parallel array of preconcentration platform
Conclusions
References
Acknowledgement
