Microarray를 이용한 일차성 자연 기흉 환자에서 유전자 발현 분석 연구
- 주제(키워드) microarray , pneumothorax , osteopontin
- 발행기관 고려대학교 대학원
- 지도교수 김광택
- 발행년도 2012
- 학위수여년월 2012. 8
- 학위구분 박사
- 학과 일반대학원 의학과
- 세부전공 흉부외과학
- 원문페이지 47 p
- 실제URI http://www.dcollection.net/handler/korea/000000034323
- 본문언어 한국어
- 제출원본 000045715912
초록/요약
Background - The pathophysiology related with the development of primary spontaneous pneumothorax (PSP) remains unclear. The purpose of our study was to identify genes potentially involved in the development of PSP. Materials and Methods - From June 2010 to December 2010, 8 consecutive patients with PSP who underwent video-assisted thoracoscopic wedge resection of lung including bullae were enrolled in this study. Lung samples were obtained for microarray analysis and real-time polymerase chain reaction (RT-PCR) from patients with primary spontaneous pneumothorax who underwent video-assisted thoracoscopic wedge resection. For two-color microarray analysis, one group of samples (Group B, bullae) was obtained from diseased lung, and the other group (Group N, normal lung tissue) was obtained from near the surgical staple line in the lung tissue of the same patient. We investigated the gene expression profiles in bullae using microarray analysis, and validated these results by RT-PCR. Immunohistochemistry was subsequently performed to identify where was highly expressed in these tissues. Results - A total of 3,706 genes were found to be up- or down-regulated in bullae using microarray analysis. Unsupervised hierarchical clustering analysis was used to identify candidate genes based on their molecular expression profile. It showed that expression levels of secreted phosphoprotein (SPP1, Osteopontin) were significantly up-regulated in blebs from patients with PSP, whereas caveolin (CAV1) was down-regulated (p<0.05). The correlation between changes in gene expression was confirmed by RT-PCR and microarray analysis for the 8 patients included in this study with bordeline significance for SPP1 expression (r =0.571, p= 0.069) and no statistical significance for CAV1 (r = 0.476, p= 0.116). SPP1 was highly expressed in alveolar macrophages in bullae, whereas low expression of SPP1 was observed in normal lung tissue. Conclusions - Our findings showed that SPP1 is related to the development of emphysema like changes (ELCs) in lung tissue, and may play an important role in the pathogenesis of PSP.
more목차
영문초록 --------------------------- i
I. 서론 ---------------------------- 1
II. 대상과 방법---------------------- 5
III. 결과---------------------------- 13
IV. 고찰---------------------------- 16
V. 요약----------------------------- 23
VI.참고문헌------------------------- 24
