The effect of magnetic nanoparticles on cell viability in magnetic field condition
- 주제(키워드) magnetic nanoparticle
- 발행기관 고려대학교 대학원
- 지도교수 안동준
- 지도교수 문대원
- 발행년도 2012
- 학위수여년월 2012. 2
- 학위구분 석사
- 학과 일반대학원 화공생명공학과
- 원문페이지 69 p
- 실제URI http://www.dcollection.net/handler/korea/000000032576
- 본문언어 한국어
- 제출원본 000045698127
초록/요약
Magnetic nanoparticles(MNPs) have been used for controlled drug delivery systems, magnetic resonance imaging. However, until now the influence of high concentrations of MNPs in magnetic field on the cell behavior in vivo have not been cleary determined yet. This study aims to investigate the behaviors of macrophage cells in wide range of concentrations in influencing magnetic field. In this study, we have prepared the polyethyleneglycol-phospholipid(PEG) coated ferrimagnetic iron oxide nanocubes(FIONs) of an average diameter of 80 nm. The cells were treated MNPs of concentrations(1.25 μg/mL ∼ 100 μg/mL) and exposed to our magnetic field device for 6 h ∼ 24 h. The cells in MNPs concentrations up to 10 μg/mL were stable state in 272.0 ∼ 69.9 mT. On the other hand, we found that the viability of cells in the 25 μg/mL of MNPs was significantly decreased comparing that of 10 μg/mL. The macrophage cells treated by 100 μg/mL MNPs showed the increase of the total cell decrement of 56 % in the magnetic field. These results could provide a novel insights into the design and synthesis of MNPs for magnetic resonance imaging contrast agents by systemically assessed cell viability.
more목차
1. 서 론 1
1.1. 연구배경 1
1.2. 산화철 자성나노입자 4
1.3. 자성나노입자의 다양한 코팅 7
1.4. 세포의 자성나노입자 uptake & cytoxicity 10
1.5. 연구목적 12
2. 실험방법 및 분석 13
2.1. 실험 재료 13
2.1.1. 시약 13
2.1.2. Cell culture dish 13
2.1.3. 산화철 자성나노입자 14
2.1.4. THP-1 cell culture & treatment 18
2.1.5. Live/Dead cell viability kit 22
2.1.6. 기기 24
2.2. Fe3O4-phospholipid-PEG 제조 25
2.3. Magnetic field device 제작 27
2.4. 형광이미지를 통한 THP-1 세포의 counting 29
3. 결과 및 고찰 31
3.1. Fe3O4-phospholipid-PEG 특성 분석 31
3.1.1. Fe3O4-phospholipid-PEG의 자성적 특성 분석 31
3.1.2. Fe3O4-phospholipid-PEG의 분산 특성 34
3.2. Magnetic field device에서의 자기력 측정 36
3.3. 세포 안에서의 자성나노입자의 위치 & 세포의 cytoxicity 확인 41
3.4. Cell viability test 44
3.4.1. 자성이 없는 조건에서의 cell viability test 44
3.4.2. 자성이 있는 조건에서의 cell viability test 47
4. 결론 53
REFERENCES 55
