(The) inhibitory effects of Rebamipide on cigarette smoke-induced airway mucin production
- 발행기관 고려대학교
- 발행년도 2004
- 학위명 석사
- 학과 및 전공 고려대학교 대학원:의학과 내과학 전공
- 식별자(기타) DL:000005719708
- 본문언어 영어
- 서지제어번호 000045134171
초록/요약 도움말
Cigarette smoke is the main cause of chronic bronchitis. Exposure of cigarette smoke induces recruitment of inflammatory cells in airway epithelium, and release of tumor necrosis factor a (TNFα) from airways. It has been reported that TNFα induces goblet cell metaplasia by activating an epidermal growth factor receptor (EGFR) cascade, resulting in mucin production. Rebamipide (2-(4-chlorobenzoylamino)-3- [2(1H)-quinolinon-4-yl] propionic acid, OPC-12759) directly inhibits the production of superoxide (O₂-) and has an inhibitory effect on proinflammatory cytokines such as TNFα and IL-8, etc. The purpose of this study is to investigate the inhibitory effects of rebamipide on cigarette smoke-induced mucin production in the airway epithelium. NCl-H292 cells and Sprague-Dawley rats were used for in vitro and in vivo studies, repectively. In vitro studies, cigarette smoke solution caused increase of TNFα secretion and EGFR-specific tyrosine phosphorylation in NCI-H292 cells, resulting in increase MUC5AC production. These effects were inhibited dose-dependently by pretreatment with rebamipide. In vivo studies, cigarette smoke caused inflammatory cell recruitment and increased secretion of TNFα in the BAL fluids, which resulted in MUC5AC production in rat airways. Rebamipide prevented recruitment of inflammatory cells and secretion of TNFα, resulting in inhibit MUC5AC production in rat airways. In conclusion, exposure of airway epithelium to cigarette smoke induces TNFa production and neutrophil recruitment, which upregulated EGFR expression, causing MUC5AC mucin synthesis. Rebamipide prevented the cigarette smoke-induced TNFa release, and MUC5AC mucin production.
more목차 도움말
Abstracts = 1
1. Introduction = 3
2. Materials and Methods = 5
3. Results = 12
4. Discussion = 15
5. Summary = 18
6. References = 20
