Continuous Separation of Different Size Micro Particles by Standing Surface Acoustic Wave (SSAW)
- 주제(키워드) SSAW , microfluidics , particle separation
- 발행기관 고려대학교 대학원
- 지도교수 Sehyun Shin
- 발행년도 2011
- 학위수여년월 2011. 2
- 학위구분 석사
- 학과 일반대학원 기계공학과
- 원문페이지 78 p
- 실제URI http://www.dcollection.net/handler/korea/000000025922
- 본문언어 영어
- 제출원본 000045642325
초록/요약
This study presents a method that uses a standing surface acoustic wave to continuously separate particles in a size-gradient manner in a microchannel flow. The proposed method was applied to a colloidal suspension containing poly-dispersed particles with three different sizes (1, 5, and 10 μm) but the same density and compressibility. Sample fluid including particles was hydrodynamically focused at an entrance region and larger particles were forced to move toward side wall, in which pressure nodes existed. To predict displacement of particles in microchannel flow, three primary forces including viscous force, acoustic radiation force and diffusion were considered and the theoretical results were compared with experimental ones. In the present study, the each different size particle was successfully separated at an optimal conditions and there existed excellent agreement between theoretical and experimental results. In addition, the present method was successfully applied to separation of cells in a size-dependent manner without any damages on cells. This study found great potential to apply the present method in many biomedical applications which require separation of cells.
more목차
Table of Contents
ABSTRACT 4
TABLE OF CONTENTS 6
LIST OF FIGURES 8
1. INTRODUCTION 9
1.1 Research Background 9
1.2 Literature Review 11
1.2.1 Particle Separation Methods 11
1.2.2 Separation Methods by Acoustic Force 13
1.2.3 Forensic DNA Analysis 15
1.2.4 Sperm Separation Methods 16
1.3 Purpose of Research 18
2. THEORY & METHODS. 19
2.1 Physics of Microfluidics 19
2.2 Surface Acoustic Waves 21
2.3 Piezoelectric Material 22
2.4 Device and Working Principle 25
3. METHODS & MATERIALS 29
3.1 Device Fabrication Process 29
3.2 Experimental Composition 30
4. RESULTS & DISSCUSION 32
4.1 Theoretical Analysis 32
4.2 Comparison of Theoretical Results with Experiments 40
4.3 Biomaterial Application for SSAW Based Cell Separation. 46
4.3.1 Experimental Setup 47
4.3.2 Micro-device Cell Separations 49
4.3.3 Post Separation DNA Analysis 52
4.3.4 Results & Discussion 53
5. CONCLUSION 63
SUPPLEMENT 65
REFERENCES 73
