Anti-obesogenic effect of delipidated soybean by up-regulation of peroxisome proliferator-activated receptor-δ in C57BL/6J and human apolipoprotein E2 transgenic mice
- 주제(키워드) obesity , PPAR , soybean
- 발행기관 고려대학교 대학원
- 지도교수 이성준
- 발행년도 2010
- 학위수여년월 2010. 8
- 학위구분 석사
- 학과 일반대학원 생명공학과
- 세부전공 식품공학전공
- 원문페이지 89 p
- 실제URI http://www.dcollection.net/handler/korea/000000023572
- 본문언어 영어
- 제출원본 000045610654
초록/요약
Soy bean, which contains high levels of dietary fibers and phytochemicals, has been suggested for its anti-obesity functions; however, the mechanism has been elusive. We examined the effect of whole component of soybean (WSB) and delipidated soybean (DLSB) on body weight reduction in male C57BL/6 mice and ApoE2 mice. Mice were fed control (AIN-76) or WSB diet (20% of casein was replaced with WSB), DLSB diet (20% of casein was replaced with DLSB) for 10 weeks. After the feeding, control group showed increase of body weight, while the body weight in WSB and DLSB groups was only marginally changed. In addition, average adipocyte size and weights were significantly decreased in WSB and DLSB group. In blood analysis, WSB and DLSB groups tended to have lower plasma concentrations of total cholesterol (TC) compare with controls; however, high density lipoprotein (HDL) / low density lipoprotein (LDL) ratio was higher in WSB and DLSB group. Insulin sensitivity of WSB and DLSB groups was also improved. Interestingly, plasma leptin content was lower while adiponectin was higher in WSB and DLSB groups compare with control. In the gene expression data, peroxisome proliferator-activated receptor-δ (PPAR-δ), uncoupling proteins (UCPs) were up-regulated while fatty acid transport protein 4 (FATP4), cluster of differentiation 36 (CD36), acyl-CoA synthetase (ACS) were down-regulated. However, Carnitine palmitoyl transferase 1 (CPT1), acyl-CoA oxidase (ACO), long-chain acyl-CoA dehydrogenase (LCAD) did not show any differences between groups. These suggest that DLSB may reduce body fat contents and adipocyte size with activation of thermogenesis and suppression of fatty acid uptake.
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CONTENTS
ABSTRACT IV
CONTENTS V
LIST OF FIGURES VIII
LIST OF TABLES X
CHAPTER 1
REVIEW OF LITERATURE １
1.1 Obesity and the metabolic syndrome １
1.2 The characteristics of peroxisome proliferator-activated receptors (PPARs) as an obesity treatment. ２
1.3 The way to reduce the body fat mass ５
1.4 The purpose of this study ６
CHAPTER 2
Effects of delipidated soy bean on weight reduction in C57BL/6J mice ９
INTRODUCTION １０
MATERIARS AND METHODS １３
2.2.1 Preparation of DLSB １３
2.2.2 Animals and diets １３
2.2.3 Blood analysis １４
2.2.4 Oral glucose tolerance test (OGTT) １５
2.2.5 Histological Analysis of Adipose Tissue １５
2.2.6 Total RNA isolation １６
2.2.7 Reverse transcription and quantitative PCR analysis １６
2.2.8 Protein isolate and immunoblot analysis １９
2.2.9 Statistical analysis １９
RESULTS ２０
2.3.1 Body weight, body fat distribution and adipocyte size. ２０
2.3.2 Biochemical parameters ２５
2.3.3 Glucose tolerance, insulin, leptin, and adiponectin ２７
2.3.4 Gene expressions ３０
2.3.5 Immunoblot analysis ３３
DISCUSSION ３６
CHAPTER 3
Effects of delipidated soy bean on weight reduction in human apolipoprtein E2 transgenic mice ４１
INTRODUCTON ４２
MATERIALS AND METHODS ４５
3.2.1 Preparation of DLSB ４５
3.2.2 Animals and diets ４５
3.2.3 Blood analysis ４７
3.2.4 OGTT ４７
3.2.5 Histological analysis of adipose tissue and liver ４７
3.2.6 Total RNA isolation ４８
3.2.7 Reverse transcription and quantitative PCR analysis ４８
3.2.8 Protein isolate and immunoblot analysis ４９
3.2.9 Statistical analysis ４９
RESULTS ５０
3.3.1 Body weight, body fat distribution and adipocyte size. ５０
3.3.2 Biochemical parameters ５６
3.3.3 Glucose tolerance, insulin, leptin and adiponectin ５８
3.3.4 Gene expression ６１
3.3.5 Immunoblot studies ６３
DISCUSSION ６６
ACNOWLEDGEMENTS ７１
REFERENCES ７２
ABSTRACT (IN KOREAN) ７８
