Fabrication Techniques and Testing of Microfluidic Biochips : Preconcentrated Electrospray Ionization Interface for Mass Spectrometry
- 주제(키워드) microfluidic , pre-concentration , electrospray , mass spectrometry , biochip , lab on a chip
- 발행기관 고려대학교 대학원
- 지도교수 김규태
- 발행년도 2010
- 학위수여년월 2010. 2
- 학위구분 석사
- 학과 일반대학원 마이크로/나노시스템협동과정
- 원문페이지 45 p
- 실제URI http://www.dcollection.net/handler/korea/000000022010
- 본문언어 영어
- 제출원본 000045592598
초록/요약
Electrospray mass spectrometry has been shown to be a suitable method for the analysis of small amounts of proteins. This study has demonstrated the feasibility of the coupling between preconcentration microfluidic device and electrospray ionization for mass spectrometry. In sequential mode the device showed high quality electrospray at 3000V with a intensity ratio of x260 and in continuous mode the device showed a x 13 intensity ratio at 2500V and both modes showed high quality electrospray suitable for mass spectrometry applications. This work suggests an example of the concept of 'Lab on a chip' and presents the possibility of multifunctional microfluidic chips. Microfabrication technologies were used to construct devices which includes preconcentration components and an electrospray tip. The tip was mechanically cut followed by a mono-layer coating process of a hydrophobic substance to minimize the dead volume of the Taylor cone. The successful coupling online of micro fabricated devices to an electrospray ionization MS represents an essential step toward the construction of automated, high-throughput, and high sensitivity analytical systems.
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