골수기질세포 및 진피섬유아세포의 창상치유능 비교 : Comparison of Human Bone Marrow Stromal Cells with Dermal Fibroblasts on Wound Healing Activity in Vitro
- 발행기관 고려대학교
- 발행년도 2005
- 학위수여년월 2005. 8
- 학위명 박사
- 학과 대학원 의학과 성형외과학전공
- 식별자(기타) DL:000015974750
- 서지제어번호 000045205326
초록/요약
Cryopreserved fibroblast implants represent an exciting medical development and a major advancement for the healing of chronic wounds. These cells may well deliver the correct growth factors at the right concentration and in the right sequence, something that has proved elusive to achieve with the topical application of recombinant growth factors. Bone marrow stromal cells, which contain mesenchymal stem cells, have a low immunity-assisted rejection rate, and they are capable of profoundly expanding their number in culture. Therefore, they appear to have several advantages over mature cells during transplantation aimed at stimulating wound healing. The purpose of this pilot study was to compare the wound healing activities of the bone marrow stromal cells with the wound healing activities of dermal fibroblasts in vitro. Human bone marrow stromal cells and dermal fibroblasts were isolated from the same patients and then grown in culture. The cells were seeded at 5.0x103 cells/well in 200㎕ of Dulbecco’s Modified Eagle Medium/Ham’s F-12 including 5% fetal bovine serum in 96-well culture plates, and they were next incubated in 5% CO2 and 100% humidity at 37℃. At 1, 3, and 5 days post-incubation, cell proliferation, collagen synthesis and secretion of basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and transforming growth factor-beta (TGF-β) were compared. Cell proliferation for the bone marrow stromal cell group was superior to the fibroblast group, with increases of 11 and 17 percent being noted at 3 and 5 days after plating, respectively (p<0.05). Collagen synthesis for the bone marrow stromal cell group was significantly increased when compared to the fibroblast group, with increases of 28, 80, and 70 percent being noted at 1, 3, and 5 days after plating, respectively (p<0.05). The bFGF levels in the bone marrow stromal cell group were also 47, 89, and 68 percent greater than the fibroblast group at each time interval (p<0.05). The most profound difference was observed in the VEGF level. The VEGF levels of the bone marrow stromal cell group were seven, twelve, and twelve fold as high as that of the fibroblast group at each time interval, respectively (p<0.05). In contrast, the TGF-β levels were significantly increased in the fibroblast group with increases of 13, 12, and 22 percent being noted at 1, 3, and 5 days after plating, respectively (p<0.05). Our results suggest that there is a surprisingly great potential of bone marrow stromal cells for wound healing, and these cells could replace the current cell therapy methods using fibroblasts.
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Abstract
I. INTRODUCTION
II. MATERIALS AND METHODS
III. RESULTS
IV. DISCUSSION
V. CONCLUSIONS
VI. REFERENCES
VII. FIGURE LEGENDS
VIII. TABLES
