Sensitivity Enhancement of Polydiacetylene Vesicles by Optimizing Vesicles Size and Polymerization Temperature
최적화된 베시클 크기와 고분자화 온도를 이용한 폴리다이아세틸렌 베시클의 민감도 성능 향상
- 주제(키워드) Polydiacetylene , Vesicles Size , Polymerization Temperature
- 발행기관 고려대학교 대학원
- 지도교수 안동준
- 발행년도 2009
- 제출일 2009-01-09
- 학위수여년월 2009. 2
- 학위명 석사
- 학과 일반대학원 화공생명공학과
- 원문페이지 89 p
- 실제URI http://www.dcollection.net/handler/korea/000000007916
- 본문언어 한국어
- 제출원본 000045534142
초록/요약
Diacetylene-funtionalized lipids can be used as the fundamental materials of effective bio-chemical sensors. Polydiacetylene vesicles change visible color from blue to red under external stimuli such as temperature, solvent, pH, mechanical stress and α-cyclodextrin. The color transition occurs when external stimuli impose stresses altering delocalization length of π-electrons in the repeated ene-yne bond moieties along polydiacetylene backbones. Most of the polydiacetylenes are prepared by 254-nm UV light of self-assembled monomers in vesicles solutions or Langmuir-Blodgett/Langmuir-Schaefer films on solid substrates. We previously investigated cyclodextrin-induced color changes in a polymerized diacetylene Langmuir-Schaefer film or vesicle solution. Cyclodextrins are well known for their ability to form inclusion complexes with various substrates. It would be useful to develop more understanding and design of PDA based chemosensors. Meantime a variety of methods to increase the sensitivity in polydiacetylene systems like variation of carbon chain length, polymerization temperature and strength of UV irradiation were reported in previous papers. Recently, it was published that smaller the vesicles are, the more sensitive is in detecting concanavalin (Con A) when polydiacetylene vesicles incorporated with glycolipids. In this study we investigated the effect of sensitivity enhancement polydiacetylene vesicles in detection α-cyclodextrin according to vesicle size by filtration technology with different pore sizes and process with different polymerization temperature. The degree of color change is quantified by UV-vis spectrometer. In order to quantify the response of polydiacetylene vesicles to the α-cyclodextrin, it can be defined colorimetric response that represents degree of color change from blue to red.
more목차
1. 서 론 1
1.1. 연구 배경 1
1.2. Diacetylene 단량체 5
1.3. Polydiacetylene (PDA) System의 색전이 현상 9
1.4. 연구 목적 12
2. 실험방법 및 분석 13
2.1. 실험 재료 13
2.1.1. 시약 13
2.1.2. Diacetylene 13
2.1.3. Liquid Analytes (Cyclodextrin) 15
2.2. 기기 18
2.2.1. UV-visible spectrophotometer 19
2.2.2. 제타 전위 측정 장치 21
2.3. Colorimetric response의 정의 23
2.4. 폴리다이아세틸렌 Vesicles의 제조 26
3. 결과 및 고찰 28
3.1. 폴리다이아세틸렌 Vesicles의 희석 효과 30
3.2. Vesicles Size 효과 35
3.2.1. Vesicles 제조 35
3.2.2. Filter Size에 따른 폴리다이아세틸렌 Vesicles 분석 35
3.2.3. Filter Size에 따른 Vesicles의 색전이 분석 38
3.2.4. Filter Size에 따른 Vesicles의 CR 분석 42
3.2.5. Filter Size에 따른 Vesicles의 경향성 분석 44
3.2.6. Vesicles의 입자 크기 46
3.2.7. Vesicles의 크기에 따른 Curvature 효과 48
3.3. 고분자화 온도 효과 50
3.3.1. Vesicles 제조 50
3.3.2. 고분자화 온도에 따른 Vesicles의 색전이 분석 53
3.3.3. 고분자화 온도에 따른 Vesicles의 CR 분석 56
3.3.4. 고분자화 온도에 따른 Vesicles 경향성 분석 58
4. 결론 67
REFERENCES 69
