Production and Characterization of Monoclonal Antibodies
- 주제(키워드) Monoclonal Antibodies
- 발행기관 고려대학교 대학원
- 지도교수 유영도
- 발행년도 2009
- 제출일 2009-01-06
- 학위수여년월 2009. 2
- 학위명 석사
- 학과 일반대학원 의학과
- 세부전공 종양생물학
- 원문페이지 28 p
- 실제URI http://www.dcollection.net/handler/korea/000000007752
- 본문언어 영어
- 제출원본 000045530826
초록/요약
Colon cancer is one of the most common malignancies in the woorld. Although surgical resection is the first choice worldwide, at this point an effective approach for the treatment of patients with metastasis and cancer recurrence post-operation has not yet been found. We report a method of inducing antigen production in SNU-16 cells by in vivo targeting that specifically bind to the SNU-C1 cells. To target SNU-16 cells, we immunized the SNU-16 gastric cancer cell to Balb/c mice. BALB/c mice were killed 3 days after the booster immunization by cervical dislocation and then a spleen cell fusion. Hybridoma clone was screening, production, purification and characterization that we found anti-SNU- C1 human colon cancer cells monoclonal antibodies.
more목차
1. INTRODUCTION
2. MATERIALS AND METHODS
2. 1. Cell culture
2. 2. Immunization
2. 3. Cell fusion
2. 4. Serum Ab determination by ELISA
2. 5. Production and purification of MAbs
2. 6. Isotype determination
2. 7. Flow cytometric detection of MAbs
2. 8. Immunohistochemical staining methods
3. RESULTS
3. 1. Generation of SNU-16-41 MAbs
3. 2. Testing of antibody specificity
3. 3. Production, Purification and characterization of SNU-16-41 MAb.
3. 4. Functional analysis of SNU-16-41 MAb.
3. 5. Binding of SNU-16-41 to human colon cancer tissue
4. DISCUSSION
5. REFERENCES
