Osteopontin-mediated phosphorylation of ERK and JNK enhances in vitro tumor invasion of human bladder cancer cells
- 주제(키워드) Osteopontin , ERK , JNK
- 발행기관 고려대학교 대학원
- 지도교수 원남희
- 발행년도 2008
- 제출일 2008-07-07
- 학위수여년월 2008. 8
- 학위명 박사
- 학과 일반대학원 의학과
- 세부전공 병리학
- 원문페이지 48 p
- 본문언어 영어
- 제출원본 000045501032
초록/요약
The major problem in the therapeutic management of bladder cancer is tumor invasion, resulting in the tumor metastasis. Although the genes orchestrating the process need to be identified, involvement of a specific gene, osteopontin (OPN), in bladder tumor invasion has to be clearly elucidated in order to understand the mechanisms of tumor invasion. Here, we show that OPN-treated cells showed increased in vitro tumor invasion. The increase correlated with activation of mitogen activated protein kinase (MAPK) and Jun NH2-terminal kinase (JNK), and the in vitro tumor invasion was inhibited by treatment with inhibitors of these kinasees. Also, the amounts of pro-inflammatory cytokines secretion were increased in OPN-treated T24 cells. In real-time PCR analysis, the expression of MMP-2 and MMP-9 mRNAs was increased, and the increase was counterbalanced by treatment with 6-aminoquinazoline (NF-kB activation inhibitor II). This novel OPN-mediated tumor invasion via cell signaling pathway plays a critical role in tumor invasion of bladder cancer cells.
more목차
1. Abstract 5
2. Introduction 7
3. Materials and Methods 10
4. Results 18
5. Discussion 23
6. Reference 30
7. Figure 36
